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CIHR Skin Research Training Centre
Core Mentors: Dr. Haishan Zeng
Haishan Zeng, PhD
Distinguished Scientist, Imaging Unit – Integrative Oncology, BC Cancer Agency
Associate Professor, Dermatology and Skin Science, UBC
Associate Member, Physics and Astronomy, UBC
Associate Member, Pathology & Laboratory Medicine, UBC

Rm 6-123, BC Cancer Research Centre, 675 West 10th Avenue, 
Vancouver, BC, V5Z 1L3 
Canada
Phone: (604) 675-8083
Fax: (604)675-8099
Website: http://www.bccrc.ca/dept/ic/cancer-imaging/haishan-zeng-phd
Email: hzeng@bccrc.ca
                                                                                                                                  

Education

1979-1983 BSc, Electronic Physics, Peking (Beijing) University
1983-1986 MSc, Electronic Physics and Devices, Institute of Electronics, Chinese Academy of Sciences
1989-1993 PhD, Biophysics, University of British Columbia 

Awards and honors

2010 Biosensors & Bioelectronics Top Cited Author for 2010 & 2011 for our paper: “Feng, S, Chen, R, Lin, J, Pan, J, Chen, G, Li, Y, Chen, M, Huang, Z, Chen, J, Zeng, H: Nasopharyngeal Cancer Detection Based on Blood Plasma Surface-Enhanced Raman Spectroscopy and Multivariate Analysis, Biosensors and Bioelectronics, 25:2414-2419, 2010.”(Impact Factor of the journal=5.602), Publisher, Analytical Chemistry & Sensors, Elsevier, Oxford, UK

2011 Best of What’s New Award (Leading inventor)- Popular Science magazine (the world’s largest science and technology magazine), Verisante’s Aura™ device for skin cancer detection 
(general info: http://www.popsci.com/bown/2011/ Award details for Verisante AuraTM: http://www.popsci.com/bown/2011/product/verisante-aura )

2011 Canadian Cancer Society’s Top 10 Breakthroughs of 2011(Dr. Haishan Zeng Team: New imaging method could improve early detection of lung cancer) (http://files.newswire.ca/1012/top10breakthroughs.pdf)

Grant Support

Canadian Institutes of Health Research
Canadian Cancer Society Research Institute
Canadian Dermatology Foundation
Etc.

Research interest

My research interests are centered on the optical properties of biological tissues and light-tissue interaction as well as their applications in medical diagnosis and therapy. Biological tissues are optically inhomogeneous and multi-layered turbid media. When light interacts with tissue, it is scattered and absorbed, and the tissue may produce fluorescence. These interactions and the optical properties of tissue determine the distribution of incoming light inside the tissue, which is important for generation of any biological effects and for therapeutic applications. On the other hand, the optical properties and the effects of tissue on light are determined by the chemical composition, morphological structure, and physiological state of the tissue. Therefore, pathological changes in tissue affect the re-emitted optical signal detected when shining a beam of light on the tissue. Through many years of research, we have demonstrated that tissue pathology can be detected in vivo by optical methods non-invasively or minimum invasively through endoscopy. Several clinically relevant devices have been successfully developed for early cancer detection in skin, lung, and gastrointestinal tract.

We are currently exploring a couple of different types of optical measurement modalities for diagnostic applications:
• Diffuse Reflectance Spectroscopy and Multi-spectral Imaging, which explores elastic light scattering and light absorption by tissue chromophores such as melanin, hemoglobin, bilirubin, and water.
• Fluorescence Spectroscopy and Imaging, which explore molecular electronic energy transition-related light emission of certain biomolecules in tissue such as tyrosine, tryptophan, NADH, collagen, and elastin.
• Raman Spectroscopy, which explores inelastic light scattering to give fingerprint-like spectral signatures of molecular vibrations. Molecules in tissue that have unique Raman signatures include proteins, DNA, lipids, glucose, and melanin.
• In-Vivo Confocal Microscopy, which provides sectional images of tissue at micron spatial resolution, allowing us to visualize cellular structures and micro-circulation in real time on living tissue. Imaging mechanisms we are exploring include elastic scattering (reflectance), two-photon excitation fluorescence (TPEF), and second harmonic generation (SHG).
• Laser Speckle Imaging, which analyzes interference patterns from light reflected off skin surfaces.

These researches may also lead to non-invasive assessment of treatment progress. We are applying optical methods to study the pharmacokinetics of photodynamic therapy (PDT) as well as to improve dosimetry for various phototherapy modalities including PDT.
In addition, we are applying MEMS (micro-electro-mechanical systems) technologies to miniaturize our optical devices for endoscopy uses and for point-of-care diagnostic applications. We are also applying nanotechnologies to enhance various light-tissue interactions for diagnostic and therapeutic applications, for example, using metal nanoparticle based SERS (surface enhanced Raman scattering) spectroscopy for diagnostics and developing nanoparticle based photo-thermal therapy.

Teaching
UBC Phys 543 - Biomedical Optics http://www.phas.ubc.ca/~hzeng/index.html
SPIE SC 823 - Diagnostic Endoscopy http://spie.org/x1145.xml?course_id=M0000892

Software
Vancouver Raman Algorithm
http://www.flintbox.com/technology.asp?page=3553 

Current lab members

Michael Short (Research Associate)
Yasser Fawzy (Research Associate)
Jianhua Zhao (Research Associate)
Anthony Lee (Postdoctoral Fellow)
Wenbo Wang (Postdoctoral Fellow)
Yingqiu Yu (Research Assistant)
Wei Zhang (Research Assistant)
Lioudmila Tchvialeva (Research Assistant)
Hequn Wang (Graduate Student)
Kam Chow (Graduate Student)
Hanna Pawluk (Graduate Student)
Yunxian Tian (Graduate Student)
Ali Majdzadeh (Undergraduate Student)

Selected recent publications

  1. Chen Y, Chen G, Zheng X, He C, Feng S, Chen Y, Lin X, Chen R, Zeng H: Discrimination of Gastric Cancer from Normal by Serum RNA based on Surface-enhanced Raman Spectroscopy (SERS) and Multivariate Analysis. Medical Physics, 39(9): 5664-5668, 2012.
  2. Wang H, Lee AMD, Frehlick Z, Lui H, McLean DI, Tang S, Zeng H: Perfectly registered multiphoton and reflectance confocal video rate imaging of in vivo human skin. Journal of Biophotonics, 2012 (Online July 2, 2012. DOI:10.1002/jbio.201200067).
  3. Yu Y, Lee AMD, Wang H, Tang S, Zhao J, Lui H, Zeng H: Imaging-guided two-photon excitation-emission matrix measurements of human skin tissues. Journal of Biomedical Optics, 17(7), 077004(1-8), 2012.
  4. Chen Y, Chen G, Feng S, Pan J, Zheng X, Su Y, Chan Y, Lin X, Lan F, Chen R, Zeng H: Label-free serum RNA analysis for colorectal cancer detection by surface-enhanced Raman spectroscopy (SERS) and multivariate analysis. Journal of Biomedical Optics, 17(6), 067003 (1-7), 2012.
  5. Wang S, Zhao J, Lui H, He Q, Zeng H: In vivo Near-infrared Autofluorescence Imaging of Pigmented Skin Lesions: Methods, Technical Improvements and Preliminary Clinical Results. Skin Research and Technology, 2012 (In press, online first doi: 10.1111/j.1600-0846.2012.00632.x).
  6. Lui H, Zhao J, Mclean DI, Zeng H: Real-time Raman spectroscopy for in vivo skin cancer diagnosis. Cancer Research, 71(10):2491-2500, 2012.
  7. Wang H, Zhao J, Lee AMD, Lui H, Zeng H: Improving Skin Raman Spectral Quality by Fluorescence Photobleaching. Photodiagnosis and Photodynamic Therapy, 2012 (In press, online first doi:10.1016/j.pdpdt.2012.02.001)
  8. Wang H, Tsai TH, Zhao J, Lee AMD, Lo BKK, Yu M, Lui H, McLean DI, Zeng H: Differentiation of HaCaT Cell and Melanocyte from their Malignant Counterparts using Micro-Raman Spectroscopy Guided by Confocal Imaging. Photodermatology, Photoimmunology & Photomedicine, 28:147-152, 2012
  9. Lin J, Chen R, Feng S, Pan J, B Li, Chen G, Lin S, Li C, Sun L, Huang Z, Zeng H: Surface-enhanced Raman scattering Spectroscopy for Potential Non-invasive Nasopharyngeal Cancer Detection. Journal of Raman Spectroscopy, 43:497-502, 2012.
  10. Lin, J., Yu, Y., Li, B., Huang, H., Lin, S., Li, C., Su, Y., Feng, S., Chen, G., Li, Y., Huang, Z., Zeng, H., Chen, R.: Electrical pulse mediated enahnced delivery of sliver nanoparticles into living suspension cells for surface enhanced Raman spectroscopy. Laser Physics Letters, 9:240-246, 2012.
  11. Chen, Y., Zheng, X., Chen, G., He, C., Zhu, W., Feng, S., Chen, G., Li, Y., Huang, Z., Zeng, H., Chen, R.: Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering. International Journal of Nanomedicine, 7:73-82, 2012
  12. Mansoor H, Zeng H, Chen K, Yu Y, Zhao J, Chiao M: Vertical optical sectioning using a magnetically driven confocal microscanner aimed for in vivo clinical imaging. Optics Express, 19:25161-25172, 2011.
  13. Wang S, Zhao J, Lui H, He Q, Zeng H: Monte Carlo simulation of near infrared autofluorescence measurements of in vivo skin. Journal of Photochemistry and Photobiology B: Biology, 105:183-189, 2011.
  14. Huang, Z., Chen, X., Chen, Y., Chen, J., Dou, M., Feng, S., Zeng, H., Chen, R.: Raman spectroscopic characterization and differentiation of seminal plasma. Journal of Biomedical Optics, 16:110501(1-3), 2011.
  15. Huang, N., Short, M., Zhao, J., Wang, H., Lui, H., Korbelik, M., Zeng, H.: Full range charaterization of the Raman spectra of organs in a murine model. Optics Express 19:22892-22909, 2011.
  16. Lee, AMD, Wang, H, Yu, Y, Tang, S, Zhao, J, Lui, H, McLean, DI, Zeng, H.: In vivo video rate multiphoton microscopy imaging of human skin, Optics Letters, 36(15):2865-2867, 2011.
  17. Lin, D, Feng, S, Pan, J, Chen, Y, Lin, J, Chen, G, Xie, X, Zeng, H, Chen, R.: Colorectoal cancer detection by gold nanoparticle based surface-enhanced Raman spectroscopy of blood serum and statistical analysis, Optics Express 19(14):13565-13577, 2011.
  18. Ye Y, Chen, Y, Li Y, Su Y, Zou C, Chen Y, Ou, L, Chen, R, Zeng, H.: Characterization and discriminatino of nasopharyngeal carcinoma and nasopharyngeal normal cell lines using confocal raman microspectroscopy. Spectroscopy - An International Journal 25:214-224, 2011.
  19. Mansoor, H, Zeng, H, Chiao, M.: Real-time thickness measurement of biological tissues using a microfabricated magnetically-driven lens actuator. Biomedical Microdevices, 13:641-649, 2011
  20. Short M, Lam, S, McWilliams, A, Ionescu, DN, Zeng, H.: Using laser raman spectroscopy to reduce false positives of autofluorescence bronchoscopies: A pilot study. Journal of Thoracic Oncology, 6:1206-1214, 2011.
  21. Chen, J, Zhuo, S, Jian, X, Zhu, X, Zheng, L, Xie, S, Lin, B, Zeng, H.: Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease. Journal of Biomedical Optics, 16(5):051305 (1-6), 2011.
  22. Lin, J, Chen, R, Feng, S, Pan, J, Li, Y, Chen, G, Cheng, M, Huang, Z, Yu, Y, Zeng, H: A Novel Blood Plasma Analysis Technique Combining Membrane Electrophoresis with Silver Nanoparticle Based SERS Spectroscopy for Potential Applications in Non-invasive Cancer Detection, Nanomedicine: Nanotechnology, Biology, and Medicine, 7:655-663, 2011.
  23. Mansoor, H, Zeng, H, Chiao, M.: A micro fabricated optical scanner for rapid non-contact thickness measurement of transparent films. Sensors & Actuators: A: Physical, 167:91-96, 2011
  24. Feng, S, Chen, R, Lin, J, Pan, J, Wu, Y, Li, Y, Chen, J, Zeng, H.: Gastric cancer detection based on blood plasma surface-enhanced Raman spectroscopy excited by polarized laser light. Biosensors and Bioelectronics 26:3167-3174, 2011.
  25. Wang, H, Huang, N, Zhao, J, Lui, H, Korbelik, M, Zeng, H.: Depth-resolved in vivo Micro-Raman Spectroscopy of a murine skin tumor model reveals cancer specific spectral biomarkers. Journal of Raman Spectroscopy 42:160-166, 2011.
  26. Lin, D, Lin, J, Wu, Y, Feng, S, Li, Y, Yu, Y, Xi, G, Zeng, H, Chen, R.: Investigation of the interactions of lymphoma cells with paclitaxel by Raman spectroscopy. Spectroscopy - An International Journal 25:23-32, 2011
  27. Yu, Y., Lin, J, Wu, Y, Feng, S, Li, Y, Huang, Z, Chen, R, Zeng, H.: Optimizing electroporation assisted silver nanoparticle delivery into living C666 cells for surface-enhanced raman spectroscopy. Spectroscopy - An Internatioanl Journal 25:13-21, 2011
  28. Guze, K., Short, M., Zeng, H., Lerman, M., Sonis, S.: Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity. Journal of Raman Spectroscopy 42:1232-1239, 2011.
  29. Wang, S, Zhao, J, Lui, H, He, Q, Zeng, H: A Modular Raman Microspectroscopy System for Biological Tissue Analysis, Spectroscopy - An International Journal, 24:577-583, 2010 (This paper was selected as the cover article)
  30. Huang, N, Wang, H, Zhao, J, Lui, H, Korbelik, M, Zeng, H: Single-wall Carbon Nanotubes Assisted Photothermal Cancer Therapy – Animal Study with a Murine Model of Squamous Cell Carcinoma, Lasers in Surgery and Medicine, 42:638–648, 2010.
  31. Feng, S, Chen, R, Lin, J, Pan, J, Chen, G, Li, Y, Chen, M, Huang, Z, Chen, J, Zeng, H: Nasopharyngeal Cancer Detection Based on Blood Plasma Surface-Enhanced Raman Spectroscopy and Multivariate Analysis, Biosensors and Bioelectronics, 25:2414-2419, 2010. (IF=5.602, 5 yr IF=5.637) (This paper has won the award of “Top Cited Author for 2010 & 2011” in Biosensors and Bioelectronics)
  32. Tchvialeva, L, Markhvida, I, Zeng, H, Lui, H, Lee, TK: Surface roughness measurement by speckle contrast under the illumination of light with arbitrary spectral profile Optics and Lasers in Engineering, Optics and Lasers in Engineering, 48:774-778, 2010.
  33. Huang, W, Pan, J, Chen, R, Li, Y, Feng, S, Xie, S, Zeng, H: Measurement of nasopharyngeal carcinoma tissue ex vivo by Raman spectroscopy. Spectroscopy and Spectral Analysis, 29:1304-1307, 2009
  34. Chen, J, Lee, A, Zhao, J, Wang, H, Lui, H, McLean, DI, Zeng, H: Spectroscopic characterization and microscopic imaging of extracted and in situ cutaneous collagen and elastic tissue components under two-photon excitation, Skin Research and Technology, 15: 418-426, 2009
  35. Feng, S, Lin, J, Cheng, M, Li, YZ, Chen, G, Huang, Z, Yu, Y, Chen, R, Zeng, H: Gold Nanoparticle Based Surface Enhanced Raman Spectroscopy of Cancerous and Normal Nasopharyngeal Tissues under NIR Laser Excitation, Applied Spectroscopy, 63(10):1089-1094, 2009 (This paper was selected as the cover article).
  36. Lin, J, Chen, R, Feng, S, Li, Y, Huang Z, Xie, S, Yu, Y, Cheng, M, Zeng, H.: Rapid delivery of silver nanoparticles into living cells by electroporation for surface-enhanced Raman spectroscopy, Biosensors and Bioelectronics, 25(2): 388-394, 2009
  37. Liao, XH, Wang, Y, Chen, R, Gu, Y, Wang, YY, Zeng, H: Monte Carlo simulation of detecting the severity of port wine stains by spectroscopy technology, Acta Photonica Sinica, 38(5): 1254-1258, 2009.
  38. Han, X., Lui, H., McLean, DI, Zeng, H.: Near-infrared autofluorescence imaging of cutaneous melanins and human skin in vivo, J. of Biomedical Optics, 14(2): 0240179(1-5), 2009.
  39. Guze, K, Short, M, Sonis, S, Karimbux, N, Chan, J, Zeng, H: Parameters Defining The Potential Applicability Of Raman Spectroscopy As A Diagnostic Tool For Oral Disease, J. of Biomedical Optics, 14 (1): 014016(1-9), 2009
  40. Cluff, GM, Short, MA, Lui, H, McLean, DI, Zeng, H, Korbelik, M, Chen, MX: Comparison of Connective Tissue Invaded by Lewis Lung Carcinoma to Healthy Connective Tissue by Means of Micro-Raman Spectroscopy, J. of Raman Spectroscopy, 40(8): 1087-1090, 2009.
  41. Chen, G., Chen, J., Zhuo, S., Xiong, S., Zeng, H., Jiang, X., Chen, R., Xie, S.: Nonlinear spectral imaging of human hypertrophic scar based on two-photon excited fluorescence and second-harmonic generation, British J. of Dermatology, 161(1): 48-55, 2009.
  42. Fawzy, Y., Zeng, H.: Intrinsic fluorescence spectroscopy for endoscopic detection and localization of the endo-bronchial cancerous lesions, J. of Biomedical Optics, 13 (6): 064022(1-8), 2008.
  43. Tchvialeva, L, Lee, TK, Markhvida, I, Mclean, DI, Lui, H. and Zeng, H.: Using a zone model to incorporate the influence of geometry on polychromatic speckle contrast, Optical Engineering, 47(7): 074201(1-6), 2008.
  44. Zeng, H, Zhao J, Short M, McLean D, Lam S, McWilliams A, and Lui H: Raman Spectroscopy for in vivo Tissue Analysis and Diagnosis – from Instrument Development to Clinical Applications. Journal of Innovative Optical Health Sciences, 1(1): 95-106, 2008.
  45. Zhao, J., Lui, H., McLean, D. I., Zeng, H.: Integrated Real-time Raman System for Clinical In Vivo Skin Analysis, Skin Research and Technology, 14: 484-492, 2008.
  46. Short M, Lam S, McWilliams A, Zhao J, Lui H, Zeng H: Development and preliminary results of an endoscopic Raman probe for potential in-vivo diagnosis of lung cancers, Optics Letters, 33(7): 711-713, 2008.
  47. Chen, R., Huang, Z., Chen, G, Li, Y., Chen, X., Chen, J., Zeng, H.: Kinetics and subcellular localization of 5-ALA induced PpIX in DHL cells via two-photon excitation fluorescence microscopy, Intl. J. Oncology, 32:861-867, 2008.

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